Characterization of a Dexamethasone-Immunosuppressed C57BL/6N Mouse Model for Chronic Cryptosporidiosis
نویسندگان
چکیده
.......... . . ...... .. . ..... .. . ..... . .. . ................................ . ............. ix INTRODUCTION ... ... ... .. . .. .... .. . ... ... .............. .. ....... ............. ....... .. .. . . . .. 1 Background ... ............ . ........ . . ... .... .. .... .............. .... . .. ...... .. ... . . ... .... 1 Statement of the Problem .. .. ....... .... ......... .. ........... . . .. . . .. .... . .... .. .... ..... 3 Purpose of this Research . ... . . .. . .. ....... . .. ....... .. .. . . .. .. . ....... . ..... .... ... . .. .. . 3 Objectives ............................................. . ................. . ... ... . .... . .. .... 3 LITERATURE REVIEW ........ . ........ ... . . ... . ... . .... .. ........ . . . ........ ...... . .. ....... 5 History . . .... . ................. .... .................. . . . .. ..... .............................. 5 Life Cycle ... ....... ... ..... . . . ....... .. ... . ........ . . . ............ ... ... .... .. ..... ... ... . . 6 Pathogenesis and Clinical Signs ... . .. ... . ........... . ................... ... . .. . ..... .. . . 8 Cryptosporidiosis in Domestic Animals ... .. . .. . . .. . . . . .. . .. . .. .. .. . .. ... . . .. . . . .... .. . 8 Cryptosporidiosis in Humans ............ ... ... .. ............. ............ ... ............ 9 Immunological Response of the Host.. ................................ .... ........... 10 In Vitro Cultivation ................ .... ..... .... .. .. .. ... .. .... . ......................... 12 In Vivo Experimentation ......................... .. ........................... ... ....... 12 Immunocompromising Agents ................ . ................ ........ ..... ..... ...... 15 Adrenal Steroids as Chemotherapeutics .. ...... . .. .. . . .. .. . ...... . ..... ... . .. .... . .. .. 18 MATERIALS and METHODS .. . . .... .. . ...... . ........ . .. ... . . . ... ....... . ... .. . ... .. ...... 22 Animals ... . ........ . ........ . .................................. . .... .... .... .... . . .. . ..... 22 Immunosuppression of Mice ............... ... ...... ............ ............ .. ......... 22 Parasites .............. . .. . . . .... ....... ..... . .. ....... .. . .. ..... ... .. ..... . ... ............ 23 Fecal Collection and Examination ................ ...... ........................... ... 24 Histological Collection and Examination ................ .. .. . . .......... .. ... . ....... 24 Immunologic Parameters .. . ... .. ......... . . ...... .. . .. .. .... .. . ... ............... .... .. 25 Splenocyte Preparation ................................. . .. .. ........... .... ... 25 B and T Lymphocyte Proliferation Assays ........... .. .................... 26 Statistical Analysis ......... ... ........ .... ........... ........ ............... ....... ... .. 27 EXPERIMENTAL DESIGN .... .. ............................. . .. . ........ . ......... . .. .. ... 29 Characterization of Chronic Cryptosporidiosis in Immunosuppressed Mice .... . 29 Effects of Cryprosporidium parvum and Dexamethasone on B and T Lymphocyte Proliferation . .. . ..... . ....... .. ................................ . .... .. 32 Effects of Dehydroepiandrosterone on Oocyst Shedding Intensities of Mice Infected with Cryptosporidium parvum .. .. ..... .. .. ......... ... . ......... .. .... 33 RES ULTS . ... . ................. . .................... .. . ............ ... .. .......... .. ........ . .. 35 Characterization of Chronic Cryptosporidiosis in Immunosuppressed Mice .... . 35 Effects of Cryptosporidium parvum and Dexamethasone on B and T Lymphocyte Proliferation .. .. ... ....... .... . .. .. . .. . .. ......... . ...... .. ... . .. .. . . . 51 Effects of Dehydroepiandrosterone on Oocyst Shedding Intensities of Mice Infected with Cryptosporidium parvum . . .. . ..... . .. .......... . ..... . . ... . .. .. .. 54 DIS CUSSIO N ............ . .. ............. . ....... . .... . ... ... .. ... ... .... .. . .. ......... .. .. . ... 59 Characterization of Chronic Cryptosporidiosis in Immunosuppressed Mice .. .. . 59 Effects of Cryptosporidium parvum and Dexamethasone on B and T Lymphocyte Proliferation ............ . ............................... . .......... . .. 70 Effects of Dehydroepiandrosterone on Oocyst Shedding Intensities of Mice Infected with Cryptosporidiumparvum ........... . ................... .. .. . ... . . 75 LITERATURE CITED ....... . .. . ....... . . . . .. . .... . .. ........... . ... . .... . ....... . . ......... 79 v
منابع مشابه
Experimental Cryptosporidium parvum infections in immunosuppressed adult mice.
Five strains of adult mice were immunosuppressed with the synthetic glucocorticosteroid dexamethasone (DEX), administered either orally or intraperitoneally. The strains of mice used were C57BL/6N, DBA/2N, CBA, C3H/HeN, and BALB/cAnN. All mice were evaluated for susceptibility to Cryptosporidium parvum after intragastric inoculation with 10(6) oocysts per mouse. The DBA/2N, CBA, C3H/HeN, and BA...
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